IN-VIVO ACUTE TOXICITY TEST OF BIOACTIVE PHYTOCHEMICALS IN LOCALLY USED MANGO AND SOURSOP LEAVES USING LABORATORY MICE

The consumption of plant-based herbal formulations have been on the increase in recent times, due to their medicinal properties, however, identification, isolation and characterization bioactive constituents for specific health therapy safety remained an issue concern. This research identified, isolated characterized succinic acid from saponin fraction Mangifera indica (mango leaf SPEM) hexamethyl cyclotrisiloxane flavonoid Annona muricata (soursop SPES). Cold maceration was used extraction with ethanol as solvent. GCMS samples mass spectra analysed gravimetric methods constituents. Results screening both revealed presence alkaloids, flavonoids, tannins, phenols, terpenoids saponins. Quantitative analysis crude ethanolic extract yielded 15.15% 20.60% respectively mango soursop leaves. selected phytochemicals high flavonoids saponin, followed by phenol tannin then alkaloids that gave least yield. Succinic Acid Hexamethyl Cyclotrisiloxane were predominant organic compounds, administered two phases a group randomly distributed mice acute toxicity test (LD50). first phase involved oral administration 10, 100 1000 mg/kg body weight, 1600, 2900 5000 weight extracts. showed fractions SPEM (Succinic Acid) found be non-toxic at doses ≤ b.w (body weight) while SPES (Hexamethyl Cyclotrisiloxane) b.w. We conclude therefore, are safe low about